Appearance (Colour)
Cream coloured
Appearance (Form)
Free flowing, homogeneous powder
Solubility
15.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 35°C - 37°C for 18- 48 hours.
Organism
Escherichia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli
ATCC 23724
Inoculum (cfu) 10-100
Growth : Good
Applications
Used for maintaining and propagating Escherichia coli in molecular microbiology procedures.
Composition
Ingredients
gm/lt.
Tryptone
10.00
Yeast extract
5.00
Directions
1. Add 15.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
