Appearance (Colour)
Light yellow to light blue
Appearance (Form)
Free flowing, homogeneous powder
Solubility
26.75 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
5.2 ± 0.2
Prepared Medium Appearance before autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Blue
Cultural Response
Inoculate and incubate at following temperatures for 18-24 hours
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth at 44°C : Good
Recovery Rate : >=50.0%
Growth at 37°C : Good
Organism
Salmonella paratyphi B ATCC 8759
Inoculum (cfu) 10-100
Growth at 44°C : Good
Recovery Rate : >=50.0%
Growth at 37°C : Good
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth at 44°C : Fair - good
Recovery Rate : 30.0 - 40.0%
Growth at 37°C : Fair - good
Organism
Escherichia coli ATCC 25922
Inoculum
Growth at 44°C : Fair
Recovery Rate : 10.0 - 20.0%
Growth at 37°C : Poor
Applications
Used for enrichment and isolation of Salmonella species.
Composition
Ingredients
gm/lt.
Papaic digest of soyabean meal
4.50
Potassium dihydrogen phosphate
1.26
Dipotassium hydrogen phosphate
0.18
Sodium chloride
7.20
Magnesium chloride
13.58
Malachite Green
0.036
Directions
1. Add 26.75 gm powder to 1.0 liter of distilled/deionize water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 115°C for 15 minutes.
