Appearance (Colour)
Cream colour
Appearance (Form)
Free flowing, homogeneous powder
Solubility
16.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 35°C - 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Indole reaction : Positive reaction
(Red ring at the interface of the medium)
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Indole reaction : Negative reaction
(No colour development/cloudy ring)
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Indole reaction : Negative reaction
(No colour development/cloudy ring)
Applications
Used for sub-cultivation for coliform differentiation and for indole testing in the bacteriological examination of water as per German Standard methods.
Composition
Ingredients
gm/lt.
Peptone
10.00
Sodium chloride
5.00
DL-Tryptophan
1.00
Directions
1. Add 16.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
