Appearance (Colour)
White to off-white
Appearance (Form)
Free flowing, homogeneous powder
Solubility
56.40 gm/liter
Solubility
Colorless,clear solution with no significane precipitate
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear with no significant precipitate
Prepared Medium Appearance after autoclaving (Colour)
Colourless
Cultural Response
Prepare the medium and dilute to 1X. Supplement with glucose as given in the directions. Incubate at 35 ± 2°C for 18-48 hours.
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 23724
Inoculum (cfu) 10-100
Growth : Good
Applications
For the cultivation of recombinant strains of Escherichia coli.
Composition
Ingredients
gm/lt.
Disodium phosphate, anhydrous
33.90
Sodium chloride
2.50
Monopotassium phosphate
15.00
Ammonium chloride
5.00
Directions
1. Add 56.40 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Autoclave at 121°C at 15 psi for 15 minutes.
4. To prepare M9 Minimal salts medium, add 200 ml M9 Minimal Salts, 5X to 750 ml sterile distilled water.
5. Cool to 45-50°C. Adjust final volume to 1.0 liter.
6. Aseptically add 20 ml of filter sterilized 20% glucose solution and, if desired 0.1 ml sterile 1.0 M Calcium chloride solution. Mix well.
7. If desired, supplement with amino acids.
