Activity
Purified PCR products and DNA fragments werepurified accourding to the kit protocol
Agarose gel electrophoresis
Purified products were tested by agarose gel electrophoresis
Description
PCR and DNA Fragment Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. The Kit utilizes a proprietary silica - based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol- chloroform extractions. The kit can be used for purification of DNA fragments of up to 10 kb using a simple and fast bind-wash-elute procedure and an elution volume of 50 μl. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/ translation without further manipulation
Includes
| Components | 50 Preps | 100 Preps |
|---|
| Spin Column | 50 Nos | 100 Nos |
| Binding Buffer | 20.0 ml | 40.0 ml |
| Wash Buffer | 75.0 ml | 150.0 ml |
| Precipitation Buffer | 40.0 ml | 80.0 ml |
| Elution Buffer | 3.0 ml | 6.0 ml |
