Appearance (Form)
Liquid
Appearance (Colour)
Blue
Appearance (Clarity)
Clear
Absence of nucleases
No DNA degradation seen when incubated overnight with Master mix.
DNA contamination
No Plasmid or Genomic DNA contamination seen
Description
Plus Mix (2X) is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase, dNTP, Mg2+ and Reaction Buffer a optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Plus Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer.
Taq Plus, a mixture of taq and pfu polymerase, blends the processivity of taq with the high fidelity of pfu. Therefore, this specially formulated Taq plus allow amplification of the higer fidelity and longer templates
than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase inmost applications. In addition, Using Taq plus results in 3´-dA overhangs PCR products, which can be used in TA clone
Applications
Long PCR with high fidelity
High reproducible PCR for complex templates
High throughput PCR for complex templates
Generation of PCR products for TA cloning.
Includes
1ml (40 rxns)
• Plus Mix (2x) - 1.0 ml
• Water, Nuclease Free - 1.0ml
5 x 1ml (200 rxns)
• Plus Mix (2x) -1.0 ml x 5
• Water, Nuclease Free - 1.0ml x 5
