Appearance (Colour)
Light cream
Appearance (Form)
Free flowing powder
Solubility
30.00 gms/lit
Solubility after autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance before autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Clear without any precipitate
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours and at 25°C for fungal * organisms
Organism
Salmonella typhimurium A ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 8739
Inoculum (cfu) 10-100
Growth : Good
Organism
Micrococcus luteus ATCC 9341
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 6538
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Candida albicans * ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Applications
For the cultivation of a variety of microorganisms.
Composition
Ingredients
gm/lt.
Pancreatic digest of casein
17.00
Dextrose
2.50
Sodium chloride
5.00
Papaic digest of soyabean meal
3.00
Dipotassium hydrogen phosphate
2.50
Directions
1. Add 30.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into test tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.