Appearance (Colour)
Yellowish white
Appearance (Form)
Free flowing, homogeneous powder
Solubility
12.60 gm/liter
Solubility before autoclaving (Clarity)
slightly opalescent may have a slight fine precipitate
pH (25°C)
8.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opalescent without significant precipitate, semi solid
Prepared Medium Appearance after autoclaving (Colour)
Colourless to whitish grey
Cultural Response
After 24-48 hours of incubation, remove the swabs aseptically and streak on appropriate medium (Tryptone Soya Agar). Incubate at 37°C for 18-24 hours with the following. After 24 hours of incubation all cultures should be viable
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Organism
Shigella dysenteriae ATCC 13313
Inoculum (cfu) 10-100
Growth : Good
Organism
Vibrio parahaemolyticus ATCC 11344
Inoculum (cfu) 10-100
Growth : Good
Organism
Vibrio cholerae ATCC 15748
Inoculum (cfu) 10-100
Growth : Good
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Organism
Neisseria meningitidis ATCC 13090
Inoculum (cfu) 10-100
Growth : Good
Gel strength
Semi-solid,comparable to 0.5% gel strength
Applications
For maintenance as a holding medium or transport medium of clinical specimens during collection or shipment.
Composition
Ingredients
gms/lt.
Sodium chloride
5.00
Disodium phosphate
1.10
Sodium thioglycollate
1.50
Agar
5.00
CaCl2 solution:
CaCl2
0.1 gm
Distilled water
10.00 ml
Directions
1. Add 12.60 gm powder to 991.0 ml distilled/purified
water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Cool to 50°C and add 9.0 ml of 1% CaCl2 solution.
4. Adjust pH to 8.4
5. Dispense into test tubes.
6. Sterilize under flowing steam for 15 minutes.
