Appearance (Colour)
Cream
Appearance (Colour)
Free flowing, homogeneous powder
Solubility
55.25 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent to opalescent, with slight precipitate
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.6 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent to opalescent, with slight precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 20° - 25°C for 40-72 hours
Organism
Vibrio fischeri ATCC 7744
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Vibrio parahaemolyticus ATCC 17802
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Applications
Used for cultivation of heterotrophic marine bacteria.
Composition
Ingredients
gm/lt.
Peptone
5.00
Yeast extract
1.00
Ferric citrate
0.10
Sodium chloride
19.45
Magnesium chloride
8.80
Sodium sulphate
3.24
Calcium chloride
1.80
Potassium chloride
0.55
Sodium bicarbonate
0.16
Potassium bromide
0.08
Strontium chloride
0.034
Boric acid
0.022
Sodium silicate
0.004
Sodium fluorate
0.0024
Ammonium nitrate
0.0016
Disodium phosphate
0.008
Agar
15.00
Directions
1. Add 55.25 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
