Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
37.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate and incubate at 44.5 °C ± 0.2 for 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Gas production : +ve
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Fair-Good
Gas production : -ve
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Gas production : +ve
Organism
Enterobacter aerogenes ATCC 13048
Inoculum
Growth : Inhibited
Organism
Bacillus subtilis ATCC 6633
Inoculum
Growth : Inhibited
Organism
Enterococcus faecalis ATCC 29212
Inoculum
Growth : Inhibited
Applications
For the differentiation and enumeration of coliforms in water, wastewater, shellfish and foods.
Composition
Ingredients
gm/lt.
Enzymatic hydrolysate of casein
20.00
Lactose
5.00
Sodium chloride
5.00
Bile salts mixture
1.50
Dipotassium phosphate
4.00
Monopotassium phosphate
1.50
Directions
1. Add 37.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into tubes containing inverted Durham’s tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
