Appearance (Colour)
Light pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
57.52 gm/liter
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Red
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Slant : A
Butt : A
Gas : (+)ve
H2S : ( -) ve
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Slant : A
Butt : A
Gas : (+)ve
H2S : ( -) ve
Gas : (+) ve
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Slant : K
Butt : A
Gas : (+) ve
H2S : ( +) ve
Gas : (+) ve
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Slant : A
Butt : A
Gas : (+ )ve
H2S : (- ) ve
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Slant : K
Butt : K
Gas : ( -)ve
H2S : (-) ve
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Slant : K
Butt : A
Gas : ( -) ve
H2S : (+) ve
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Slant : K
Butt : A
Gas : ( -) ve
H2S : (- ) ve
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Slant : K
Butt : A
Gas : (-)ve
H2S : (+)ve
Organism
Salmonella Paratyphi A ATCC 5006
Inoculum (cfu) 10-100
Growth : Good
Slant : K
Butt : A
Gas : (+) ve
H2S :(-)ve
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Slant : K
Butt : K
Gas : (+) ve
H2S : (+) ve
Key
A = acidic, yellow; K = alkaline, no change; Gas = (+) splitting and cracking of the medium, Gas = (-) no splitting and cracking of the medium, H2S = (+) black precipitate; H2S = (-) no black precipitate
Applications
For the differentiation and identification of gram-negative bacilli based upon the fermentation of dextrose and lactose and production of H2S.
Composition
Ingredients
gm/lt.
Veg. Peptone
15.00
Veg. Extract
3.00
Yeast extract
3.00
Veg. Peptone No.3
5.00
Sodium chloride
5.00
Dextrose
1.00
Ferrous sulphate
0.20
Sodium thiosulphate
0.30
Phenol Red
0.024
Lactose
10.0
Agar
15.00
Directions
1. Add 57.52 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into test tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
