Appearance (Colour)
Yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
39.50 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Yellow, after addition of 5.0 -7.0% lysed blood forms reddish brown colored opalescent gel.
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24 - 48 hours with the following :
Organism
Campylobacter fetus subsp.jejuni ATCC 29428
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50.0%,grey -brown colonies
Organism
Campylobacter jejuni ATCC 33560
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50.0%,grey -brown colonies
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth* : None-poor
Organism
Streptococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth* : None-poor
Organism
Escherichia coli ATCC 8739
Inoculum (cfu) 10-100
Growth* : None- Poor
Key
* after addition of Campylobacter Supplement I (Blaser - Wang)
** after addition of Campylobacter Supplement II (Skirrow).
Applications
Used with addition of blood and antibiotics in isolation and cultivation of Campylobacter species.
Composition
Ingredients
gm/lt.
Proteose peptone
15.00
Sodium chloride
5.00
Liver digest
2.50
Yeast extract
5.00
Agar
12.00
Directions
1. Add 39.50 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C and aseptically add 5.0 -7.0% sterile lysed horse blood or 10% sterile defibrinated sheep blood and Campylobacter Supplement I (Blaser - Wang) or Campylobacter Supplement II (Skirrow).
5. Mix well and dispense into sterile petri plates.
