Appearance (Clarity)
Light yellow with bluish green tint
Appearance (Colour)
Free flowing, homogeneous powder
Solubility
20.33 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Greenish blue
Cultural Response
Inoculate and incubate at 37° C for 18 - 48 hours
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Green/Blue, Gas production : Negative
Organism
Salmonella typhimurium ATCC 6539
Inoculum (cfu) 10-100
Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Applications
Used to distinguish between anaerobic and aerobic breakdown of carbohydrate (Dextrose).
Composition
Ingredients
gm/lt.
Peptone
2.00
Sodium chloride
5.00
Dipotassium phosphate
0.30
Dextrose
10.0
Bromothymol Blue
0.03
Agar
3.00
Directions
1. Add 20.33 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
