Appearance (Colour)
Cream to yellow coloured
Appearance (Form)
Free flowing, homogeneous powder
Solubility
23.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 35°C - 37°C for 18-24 hours
Organism
Bacillus cereus ATCC 10876
Inoculum (cfu) 10-100
Growth : Good
Motility : Positive
Organism
Bacillus anthracis ATCC 14578
Inoculum (cfu) 10-100
Growth : Good
Motility : Negative
Organism
Bacillus thuringiensis ATCC 14578
Inoculum (cfu) 10-100
Growth : Good
Motility : Positive
Applications
Used for enumeration of motility of Bacillus cereus strains.
Composition
Ingredients
gm/lt.
Casein enzymic hydrolysate
10.00
Dextrose
5.00
Yeast extract
2.50
Disodium phosphate
2.50
Agar
3.00
Directions
1. Add 23.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense in tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5 .Solidify tubes in upright position.
