Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Activity
One unit is defined as the amount of enzyme required to digest 1 µg of lambda DNA in 1 hour at 37 °C in 50 µL of assay buffer.
DNA degradation
Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C.
Description
SacI is an Isoschizomers of SstI,
Neoschizomers: Ecl136II, EcoICRI
Source: Streptomyces Stanford
5'-G AGCT↓C-3'
3'-C↑TCGA G-5'
Includes
750U
SacI - 75 μl
10X Reaction Buffer – 1.0ml
1250U
SacI - 125 μl
10X Reaction Buffer – 1.0ml