Appearance (Colour)
Creamish tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
55.15 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
6.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Lactobacillus leichmannii ATCC 7830
Inoculum (cfu) 10-100
Growth : Good
Recovery : >= 70.0%
Organism
Lactobacillus plantarum ATCC 8014
Inoculum (cfu) 10-100
Growth : Good
Recovery : >= 70.0%
Organism
Lactobacillus fermentum ATCC 9338
Inoculum (cfu) 10-100
Growth : Good
Recovery : >= 70.0%
Applications
Used for the cultivation of lactobacilli.
Composition
Ingredients
gm/lt.
Veg .Peptone
10.00
Veg. Extract
10.00
Yeast extract
5.00
Dextrose
20.00
Polysorbate 80
1.00
Ammonium citrate
2.00
Sodium acetate
5.00
Magnesium sulphate
0.10
Manganese sulphate
0.05
Dipotassium phosphate
2.00
Directions
1. Add 55.15 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
