Appearance (Colour)
Light yellow to pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
58.0 gm/liter
Gel strength (1.5% gel)
Firm, comparable with 1.25% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Orange
Cultural Response
Inoculate and incubate at 22-32°C for 24-48 hours
Organism
Yersinia enterocolitica ATCC 27729
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=50.0%
Colony characteristics : translucent with dark pink centre and bile precipitate
Organism
Escherichia coli ATCC 25922
Inoculum
>=10³
Growth : Inhibited
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=10³
Growth : Inhibited
Applications
For the isolation of Yersinia enterocolitica from clinical specimens and food samples.
Composition
Ingredients
gms/lt.
Special peptone
20.00
Yeast extract
2.00
Mannitol
20.00
Sodium deoxycholate
0.50
Sodium chloride
1.00
Sodium pyruvate
2.00
Magnesium sulphate
0.01
Neutral Red
0.03
Crystal Violet
0.001
Agar
12.50
Directions
1. Add 58.00 gm powder to distilled /purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15psi pressure at 121°C for 15 minutes.
5. Cool to 45 -50°C.
6. Aseptically, add reconstituted contents of 1 vial of Yersinia Selective Supplement (15061).
