Appearance (Colour)
Light biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
30.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours or upto 7 days in case of fungi*.
Organism
Candida albicans * ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 20 - 25°C
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Clostridium sporogenes ATCC 19404
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Micrococcus luteus ATCC 9341
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Applications
A nutritious general purpose medium used for the growth of a wide variety of bacteria and fungi.
Composition
Ingredients
gm/lt.
Papaic digest of soybeanmeal
3.00
Dipotassium phosphate
2.50
Enzymatic digest of casein
17.00
Sodium chloride
5.00
Glucose
2.50
Directions
1. Add 30.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes or as desired.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
