Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
36.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
After addition of blood : Chocolate brown, opaque
Cultural Response
Inoculate and incubate at 37°C for 24 hours
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pneumoniae ATCC 6303
Inoculum (cfu) 10-100
Growth : Good
Applications
For the isolation and identification of a variety of fastidious microorganisms.
Composition
Ingredients
gm/lt.
Agar
10.00
Sodium chloride
5.00
Corn starch
1.00
Proteose peptone no. 3
15.00
Di potassium phosphate
4.00
Monopotassium phosphate
1.00
Directions
1. Add 36.00 gm powder to 990 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. Add aseptically 10 ml of sterile defibrinated sheep blood.
6. Gently heat while stirring and bring to 85°C for 5-10 minutes.
7. Cool to 45-50°C.
8. Dispense into sterile petri plates.
