Appearance (Colour)
Beige
Appearance (Form)
Free,flowing ,homogenous powder
Solubility
52.0 gms/lit.
pH (25°C)
7.60 - 7.90
Prepared Medium Appearance after autoclaving (Colour)
Light amber to medium amber
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm,comparable with 1.5% agar gel
Cultural Response
Inoculate and incubate at 37°C for 18- 24 hourswith the following.
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
PYR (on addition of PYR reagent) :Positive,red colouration
Organism
Enterococcus faecali ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
PYR (on addition of PYR reagent) :Positive,red colouration
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
PYR (on addition of PYR reagent) : Negative
Applications
Used for the isolation and identification of Streptococcus pyogenes.
Composition
Ingredients
gm/lt.
Beef heart, infusion from(ex.buffalo)
500.00
Peptone
20.00
Sodium chloride
2.00
Dextrose
2.00
Disodium hydrogen phosphate
0.40
Sodium carbonate
2.50
Chromogenic mixture
0.10
Agar
15.00
Directions
1. Add 52.0 gms powder in 1000 ml distilled/purified water.
2. Gently heat and bring to boiling.
3. Autoclave at 15 lbs pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.Mix well and pour in petriplates.
