Appearance (Colour)
Light beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
45.00 gm/liter
Solubility before autoclaving (Clarity)
Opalescent with a flocculent precipitate
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opalescent with a flocculent precipitate
Prepared Medium Appearance after autoclaving (Colour)
Very light to light amber
Cultural Response
Inoculate and incubate at 30 ± 2°C for 18-48 hours
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >= 50%, Colony morphology : smooth, circular brown to black, no colour diffusion, no sheen
Organism
Escherichia coli ATCC 25922
Inoculum
>=103, Growth : Inhibited
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=103, Growth : Inhibited
Organism
Candida tropicalis ATCC 750
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >= 50%, Colony morphology : smooth,discrete, dark brown w/black centers, black diffusion after 72 hours, sheen slight, mycelial fringe
Applications
For the detection and isolation of Candida species.
Composition
Ingredients
gm/lt.
Agar
16.00
Glycine
10.00
Glucose
10.00
Bismuth ammonium citrate
5.00
Sodium sulphite
3.00
Yeast extract
1.00
Directions
1. Add 45.00 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave or overheat.
4. Dispense into sterile petri plates.
