Appearance (Form)
Liquid
Appearance (Colour)
Blue
A260/A280
1.7~1.9
Concentration
130-150ng/µl
Stability
No obvious degradation was seen after incubation at 37°C for 1 week. Well-defined bands are formed during agarose gel electrophoresis.
Description
The SafeDye 1kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments. The 500bp and 3,000bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. BioLit™ SafeDye emits green fluorescence when bound to dsDNA, ssDNA and RNA. This stain has a fluorescence excitation maxima when bound to nucleic acid at approx. 290-320nm and emits at 490nm. This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel.
Includes
• SafeDye 1Kb Ladder Plus - 50 mcg
• SafeDye Stain - 250 mcl