Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
46.18 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent .
pH (25°C)
6.7 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent, may have a slight precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18-48 hours
Organism
Lactobacillus viridescens ATCC 12706
Inoculum (cfu) 10-100
Growth : Good
Organism
Lactobacillus acidophilus ATCC 4356
Inoculum (cfu) 10-100
Growth : Good
Organism
Leuconostoc mesenteroides ATCC 12291
Inoculum (cfu) 10-100
Growth : Good
Applications
Used for the cultivation of heterofermentative lactobacilli requiring high thiamine content.
Composition
Ingredients
gm/lt.
Tryptone
12.50
Yeast extract
7.50
Dextrose
10.00
Sodium citrate
5.00
Sodium chloride
5.00
Dipotassium phosphate
5.00
Magnesium sulphate
0.80
Manganese chloride
0.14
Ferrous sulphate
0.04
Polysorbate 80
0.20
Thiamine hydrochloride
0.001
Directions
1. Add 46.18 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
