Appearance (Colour)
Yellow
Appearance (Form)
Crystalline powder
Solubility (Turbidity) 5% solution in Methanol
Clear
Solubility (Colour) 5% solution in methanol
Yellow
Assay
min. 98 %
Melting Point
239 - 241°C
Sulphated Ash
max. 0.1 %
Application
Ellman"s Reagent or DTNB, this was originally developed for use as a sulfhydryl reagent. It can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410nm). Only protein thiols that are accessible to this water-soluble reagent are modified.