Appearance (Colour)
White to light pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
23.27 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 25-30°C for upto 7 days
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth (Plain):- Poor>br>Recovery w/hydrocarbons:- Good
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth (Plain):- Poor
Recovery w/hydrocarbons:- Good
Applications
For examining fuels for microbial contamination and for studying hydrocarbon utilization by microorganisms.
Composition
Ingredients
gm/lt.
Magnesium sulphate
0.20
Calcium chloride anhydrous
0.02
Monopotassium phosphate
1.00
Dipotassium phosphate
1.00
Ammonium nitrate
1.00
Ferric chloride
0.05
Agar
20.00
Directions
1. Add 23.27 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Dispense into sterile petri plates.
?Note : A white precipitate prior to sterilization becoming yellow to orange after sterilization is normal.
