Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light yellow ( Note : If desired add defibrinated blood After addition of 5% sterile defibrinated blood: Cherry red, opaque.)
Cultural Response
Inoculate and incubate at 30 ± 2°C for 18-24 hours
Organism
Candida albicans* ATCC 10231
Inoculum (cfu) 10-100
Growth w/blood : Good
Growth w/o blood : Good
Heamolysis : ---
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth w/blood : Good
Growth w/o blood : Good
Recovery Rate : >=70.0%
Haemolysis : Beta
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth w/blood : Good
Growth w/o blood : Good
Recovery Rate : >=70.0%
Haemolysis : Beta
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth w/blood : Good
Growth w/o blood : Good
Recovery Rate : >=70.0%
Haemolysis : None
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth w/blood : Good
Growth w/o blood : Good
Recovery Rate : >=70.0%
Haemolysis : None
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth w/blood : Good
Growth w/o blood : Good
Recovery Rate : >=70.0%
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth w/blood : Good
Growth w/o blood : Good
Recovery Rate : >=70.0%
Applications
Used for the isolation and cultivation of fastidious & non fastidious microorganisms.
Composition
Ingredients
gm/lt.
Papaic digest of soybeanmeal
15.00
Agar
15.00
Pancreatic digest of casein
15.00
Sodium chloride
5.00
Directions
1. Add 40.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
