Appearance (Colour)
Grey to black
Appearance (Form)
Free flowing homogeneous powder
Solubility
24.00 gms/lit
pH (25°C)
7.20 - 7.60
Prepared Medium Appearance after autoclaving (Colour)
Black
Prepared Medium Appearance after autoclaving (Clarity)
opaque gel
Gel strength
Firm,comparable with 0.3% agar gel
Cultural Response
Inoculate and incubate at 25 -30°C for 5 days,and further subculturing on Tryptone Soya Agar.
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Vibrio cholerae ATCC 15748
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Organism
Neisseria gonorrhoea ATCC 43069
Inoculum (cfu) 10-100
Growth : Good
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Applications
For transportation of clinical specimens.
Composition
Ingredients
gms/lt.
Sodium thioglycolate
0.90
Charcoal
10.00
Sodium b glycerophosphate
10.00
Calcium chloride anhydrous
0.10
Methylene Blue
0.002
Agar
3.00
Directions
1.Add 24.00 gms powder to 1.0 litre distilled /purified water and mix thoroughly.
2. Gently heat and bring to boiling. Dispense in tubes with constant strinng to evenly suspnd charcioal particles
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool the tubes in upright position.
