Appearance (Form)
Liquid
Appearance (Colour)
Blue
A260/A280
1.7~1.9
Concentration
~100ng/µl
Stability
No obvious degradation was seen after incubation at 37°C for 1 week. Well-defined bands are formed during agarose gel electrophoresis.
Description
The SafeDye 100bp ladder are ideal for determining the size of double-stranded DNA from 100 to 1500 base pairs. The ladder consists of 11 linear double-stranded fragments .The 500bp fragment is present at increased intensity to allow easy identification.. All fragments are precisely quantified and mixed during the production. For 5 mcl loading, all fragments except 500bp are 40ng. The 500bp fragment is 100ng.BioLit™ SafeDye emits green fluorescence when bound to dsDNA, ssDNA and RNA. This stain has a fluorescence excitation maxima when bound to nucleic acid at approx. 290-320nm and emits at 490nm. This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel.
Includes
• SafeDye 100bp Ladder - 50 mcg
• SafeDye Stain - 250 mcl
