Appearance (Colour)
White to off white
Appearance (Form)
Crystalline powder
Solubility (Turbidity) 0.1% aq. solution
Clear
Solubility (Colour) 0.1% aq. solution
Colourless to pale yellow
Activity
min. 40 BTEE units/mg protein
min. 7500 ATEE units/mg powder
min. 1000 USP units/mg powder
Protein content
min. 70%
Unit Definition
1) One unit of α chymotrypsin will hydrolyze 1.0 µmole of BTEE per minute at pH 7.8 at 25°C (1 BTEE unit = 29.5 NF/USP unit).
2) One unit of α chymotrypsin will hydrolyze 1.0 micromole of ATEE per minute at pH 7.0 at 25 °C (1 NF/USP unit = 7.5 ATEE units).
Enzyme solution for activity determination
Prepared in 0.001 N HCl
