Appearance (Colour)
White, fine powder
Solubility
Soluble in distilled water
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours (Aseptically,add the rehydrated contents of 1 vial of Streptococcus Selective Supplement (35140) as per direction)
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Haemolysis : Beta
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Good
Haemolysis : Gamma
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Haemolysis : Beta
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Haemolysis : None
Organism
Clostridium perfringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Haemolysis : None
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation and cultivation of Streptococcus species.
Composition
Colistin sulphate
5.0 mg/vial
Oxolinic acid
2.5 mg/vial
Directions
1. Aseptically add 2 ml of sterile distilled water to 1 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and dispense as desired.
3. Add 2 ml (1 vial) of supplement to 500 ml of Columbia Blood Agar Base w/1% Agar.
