Appearance (Colour)
Cream to yellow
Appearance (Form)
Free, flowing homogeneous powder
Solubility
29.60 gms/lit.
Solubility before autoclaving (Colour)
Light amber
Solubility before autoclaving (Clarity)
opalescent
Gel strength
Firm,comparable with 2.0% agar gel
pH (25°C)
7.0 - 7.40
Prepared Medium Appearance after autoclaving (Clarity)
opaque gel with whte precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 35-37°C for 24 - 48 hours with the following organisms
Organism
Potassium solubilizing isolate
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Potassium solubilization : Positive reaction,clear zone surrounding the colony
Applications
Widely used media for isolation and identification of Potassium solubilizing bacteria from soil samples.
Composition
Ingredients
gms/lt.
Magnesium sulphate
0.50
Calcium carbonate
0.10
Potassium alumino silicate
2.00
Dextrose
5.00
Ferric chloride
0.005
Calcium phosphate
2.00
Agar
20.00
Directions
1. Add 29.60 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
