Appearance (Colour)
White, fine powder
Solubility
Soluble in distilled water
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours (Aseptically,add the rehydrated contents of 1 vial of Polymyxin B selective supplement (35980) Growth observed on MYP Agar Base.
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Yellow
Lecithinase : Negative
Organism
Bacillus cereus ATCC 10876
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Red
Lecithinase : Positive
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Yellow
Lecithinase : Positive
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : None to poor
Colony colour : None
Lecithinase : Negative
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : None to poor
Colony colour : None
Lecithinase : Negative
Applications
Used for the selective isolation of various microorganisms.
Composition
Polymixin B Sulphate
50,000 units/vial.
Directions
1. Aseptically add 2ml of sterile distilled water to 1 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and pour in petriplates.
i. Add 2 ml (1 vial) of Polymixin B Selective Supplement to 200 ml of Salt Polymixin Broth Base or
ii. Add 2 ml (1 vial) of Polymixin B Selective Supplement to 450 ml of MYP Agar Base or Modified MYP Agar Base.
iii. Add 2 ml (1 vial) of Polymixin B Selective Supplement to 500 ml of Bacillus Cereus Agar Base, KG Agar Base, Salt Polymixin Broth Base or SDS Agar.
