Appearance (Form)
Liquid
Appearance (Colour)
Blue
Appearance (Clarity)
Clear
Absence of nucleases
No DNA degradation seen when incubated overnight with the Master mix.
DNA contamination
No Plasmid or Genomic DNA contamination seen.
Description
2X Long Taq Mix is a premixed, ready-to-use solution containing Long Taq DNA Polymerase, dNTP, MgCl2 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. Long taq DNA Polymerase, a combination of two thermostable DNA polymerases - Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.
Long taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long Taq in your PCR reactions results in 3´-dA overhangs PCR products, which can be used in TA clone.
Applications
PCR for long templates of up to 40kb
High reproducible ,high throughput PCR for complex template
Includes
1ml (40 rxns)
• Long Taq Mix (2x) - 1.0 ml
• Water, Nuclease Free - 1.0ml
5 x 1ml (200 rxns)
• Long Taq Mix (2x) - 1.0 ml x 5
• Water, Nuclease Free 1.0ml x 5
