Composition
Tryptone Broth - 62600
MRVP Medium - 14833
Simmons Citrate Agar - 59437
Tryptone Broth - 62600
Dehydrated Appearance
Light cream, free flowing, homogeneous powder
Solubility
15.00 gm/litre
Prepared Medium Appearance
Light amber,clear
pH (25°C)
7.5 ± 0.2
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Indole production : (+)ve
Organism
Enterobacter aerogenes
ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Indole production : (-)ve
Key
(+)ve --- formation of red-pink colour at the interface of
medium
(-)ve ---no formation of red-pink colour at the interface
of medium
MRVP Medium - 14833
Dehydrated Appearance
Cream, free flowing, homogeneous powder
Solubility
17.00 gm/ litre
Prepared Medium Appearance
Light amber,clear
pH (25°C)
6.9 ± 0.2
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
MR test : (+) ve
VP test : (-) ve
Organism
Enterobacter aerogenes
ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
MR test : (-) ve
VP test : (+) ve
Key
Methyl red test :
(+) ve : bright red colour
(-) ve : yellow-orange colour
Voges-Proskauer test :
(+) ve : red colour
(-) ve : no red colour
Simmons Citrate Agar - 59437
Dehydrated Appearance
Mustard yellow, free flowing, homogeneous powder
Solubility
24.28 gm/liter
Prepared Medium Appearance
Forest green, clear to slightly opalescent
pH (25°C)
6.9 ± 0.2
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Salmonella typhi
ATCC 6539
Inoculum
Growth : Fair-good
Citrate utilization : (-) ve
Organism
Enterobacter aerogenes
ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Citrate utilization : (+) ve
Key
(+) ve : blue colour
(-) ve : no change in colour,green
Applications
Used for biochemical identification of gram negative enteric bateria based on Indole production, Methyl Red and Voges Proskauer reactions and Citrate Utilization test.
