Appearance (Colour)
Cream, free flowing, homogeneous powder
Solubility
28.7 gm/500ml
Solubility before autoclaving (Clarity)
Clear solution with very slight precipitate
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear solution with very slight precipitate
Prepared Medium Appearance after autoclaving (Colour)
Medium amber to amber (without addition of supplement)
Medium amber to yellow clear solution with very slight precipitate (after addition of supplement)
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48 hrs
Organism
Listeria monocytogenes
ATCC 19118
Inoculum (cfu) 10-100
Growth : Good,
Esculin Hydrolysis : +( postive, blackening of medium)
Organism
Pseudomonas aeruginosa
ATCC 27853
Inoculum (cfu) 10-100
>=103
Growth : Inhibited
Esculin Hydrolysis : -(negative, no blackening of medium)
Organism
Listeria monocytogenes
ATCC 19117
Inoculum (cfu) 10-100
Growth : Good,
Esculin Hydrolysis : +( postive, blackening of medium)
Organism
Escherichia coli
ATCC 25922
Inoculum
>=103
Growth : Inhibited
Esculin Hydrolysis : -(negative, no blackening of medium)
Applications
Recommended selective, enrichment medium for the isolation of Listeria species.
Composition
Ingredients
gm/lt.
Proteose peptone
5.00
Tryptone
5.00
Beef extract(ex.buffalo)
5.00
Yeast extract
5.00
Sodium chloride
20.00
Disodium hydrogen phosphate
12.00
Potassium dihydrogen phosphate
1.35
Esculin
1.00
Lithium chloride
3.00
Directions
1. Add 28.7 gm powder to 500ml distilled/purified water.
2. Mix well to dissolve completely.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C & add aseptically add the contents of 1 vial of Fraser Selective Supplement (52071) or as desired.
Note : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours.
