Appearance (Colour)
Beige
Appearance (Form)
Homogeneous,free flowing powder.
Solubility
63.5 gms/lit.
Solubility before autoclaving (Colour)
Light to medium amber
Solubility before autoclaving (Clarity)
Clear to slightly opalescent.
Prepared Medium Appearance
Light to medium amber,clear to very slightly opalescent.
Gel strength
Firm,comparable with 1.5% agar.
Cultural Response
Cultural Response : Inoculate and incubate at 35-37°C for 18 -48 hours.
Organism
Lactobacillus casei ATCC 9595
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Organism
Lactobacillus lactis ATCC 8000
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Organism
Streptococcus thermophilus ATCC 14485
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=50.0%
Applications
Recommended for enumeration and identification of lactic Streptococci and Lactobacilli by pour plate method.
Composition
Ingredients
gm/lt.
Enzymatic digest of casein
20.00
Yeast extract
5.00
Gelatin
2.50
Sodium chloride
4.00
Dextrose
5.00
Lactose
5.00
Saccharose (Sucrose)
5.00
Sodium acetate
1.50
Ascorbic acid
0.50
Agar
15.00
Directions
1. Add 63.50 gms powder to 1.0 liter distilled water/deionised water.
2. Gently heat and bring to boiling.
3. Autoclave at 121°C at 15 psi pressure for 15 minutes.
