Appearance (Colour)
Light cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
30.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
pH (25°C)
8.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 48 hours
Organism
Clostridium perfringens ATCC 12924
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Organism
Micrococcus luteus ATCC 10240
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Fair-good
Applications
Recommended medium for cultivation of anaerobes on large scale, especially for vaccine manufacturing.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
10.00
Liver tissues, infusion from
250.00
Muscle tissues, infusion from
250.00
Sodium chloride
5.00
Sodium thioglycolate
1.00
Dipotassium phosphate
4.00
Directions
1. Add 30.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. If desired, adjust pH to 8.4.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Cool to 45-50°C, aseptically add 0.5% sterile dextrose solution if required.
