Appearance (Colour)
Beige with pink tint
Appearance (Form)
Free flowing, homogeneous powder
Solubility
67.24 gms/litre
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent gel
Prepared Medium Appearance after autoclaving (Colour)
Red
Cultural Response
Inoculate and incubate at 30-37 °C for 24 - 48 hours
Organism
Listeria monocytogenes ATCC 19118
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Bluish -green
Rhamnose Fermentation : Positive reaction (yellow halo)
Organism
Listeria innocua ATCC 33090
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Bluish -green
Rhamnose Fermentation : Positive reaction (yellow halo)
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum
Growth : Inhibited
Applications
For the selective identification and differentiation of Listeria monocytogenes.
Composition
Ingredients
gm/lt.
Peptone, Special
23.00
Sodium chloride
5.00
Yeast extract
1.00
Meat extract
5.00
Lithium chloride
5.00
Rhamnose
10.00
Phenol Red
0.12
Chromogenic mixture
5.13
Agar
13.00
Directions
1. Add 33.62 gm powder to 500.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. If desired, aseptically add 1 vial of Listeria Selective Supplement. Mix well.
