Appearance (Colour)
Pinkish biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
42.53 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Reddish orange
Cultural Response
Inoculate and incubate at 37°C for 18- 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=50.0%,
Colony colour : Pink w/bile precipitate
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good,
Recovery Rate :>=50.0%,
Colony colour : Pink may have slight bile precipitate
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=50.0%,
Colony colour : Colourless
Organism
Bacillus subtilis ATCC 6633
Inoculum
>=10³
Growth : Inhibited
Organism
Enterococcus faecalis ATCC 29212
Inoculum
>=10³,
Growth : Inhibited
Applications
For the isolation and differentiation of Gram-negative enteric bacilli and for enumeration of coliforms from water, waste water, milk and dairy products.
Composition
Ingredients
gms/lt.
Peptone, special
10.00
Agar
15.00
Lactose
10.00
Sodium chloride
5.00
Sodium citrate
2.00
Sodium deoxycholate
0.50
Neutral Red
0.033
Directions
1. Add 42.53 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Do not autoclave or overheat.
