Appearance (Colour)
Pale green to green
Appearance (Form)
Free flowing, homogeneous powder
Solubility
26.62 gms/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
5.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Blue, may have presence of slight precipitate
Cultural Response
Inoculate and incubate at 42-43°C for 18-48 hours. After incubation ,subculture on Brilliant Green Agar (65774)/MacConkey agar (76875) and incubate at 35 ± 2°C for 18-24 hours
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50.0%
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50.0%
Organism
Escherichia coli ATCC 25922
Inoculum
>=103,Growth : Inhibited
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=50.0%
Applications
Used for the selective enrichment of Salmonella from meat and dairy products, faeces and sewage polluted water.
Composition
Ingredients
gm/lt.
Enzymatic digest of casein
4.54
Magnesium chloride anhydrous
13.40
Sodium chloride
7.20
Potassium Dihydrogen phosphate
1.45
Malachite Green oxalate
0.036
Directions
1. Add 26.62 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 115 -116°C for 15 minutes.
