Appearance (Colour)
Pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
59.09 gm/liter
Solubility before autoclaving (Clarity)
Very slightly to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Greenish brown
Cultural Response
Inoculate and incubate plates at 35 ± 2°C for 24-48 hours
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : Pink white
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : None - poor
Colony colour : Yellow green w/intense yellow - green zone
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Inhibited
Colony colour : -
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Inhibited
Colony colour : -
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate >=50.0%
Colony colour : Pink white w/brilliant red zone
Applications
For the selective isolation of Salmonella species, other than Salmonella typhi from food, dairy products, eggs, egg products and feed.
Composition
Ingredients
gm/lt.
Yeast extract
3.00
Proteose peptone no. 3
10.00
Lactose
10.00
Sucrose
10.00
Sodium sulfapyridine
1.00
Sodium chloride
5.00
Agar
20.00
Brilliant Green
0.0125
Phenol Red
0.08
Directions
1. Add 59.09 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Do not overheat .
