Appearance (Form)
Light cream
Appearance (Form)
Free flowing, homogenous powder
Solubility
13.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Applications
An animal origin free media, for the cultivation and maintenance of a wide variety of microorganisms .
Composition
Ingredients
gm/lt.
Veg. Peptone
5.00
Sodium chloride
5.00
Yeast extract
1.50
Veg. extract
1.50
Directions
1. Add 13.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.