Appearance (Colour)
Pinkish yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
60.25gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Reddish orange
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%, Colony colour : Colourless with black centres
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%, Colony colour : Colourless with black centres
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=30%, Colony colour : Pink with bile precipitate
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=40%, Colony colour : Colourless may have black centres
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=40%, Colony colour : Colourless
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=30%, Colony colour : Cream pink
Organism
Enterococcus faecalis ATCC 29212
Inoculum
Growth : Inhibited
Applications
For the selective isolation and differentiation of pathogenic enteric bacilli especially Salmonella and Shigella species.
Composition
Ingredients
gm/lt.
Peptone
2.50
Agar
13.50
Lactose
10.00
Sodium thiosulphate
8.50
Sodium citrate
8.50
Beef extract(ex. buffalo)
5.00
Bile salts
8.50
Tryptone
2.50
Ferric citrate
1.00
Brilliant Green
0.00033
Neutral Red
0.025
Directions
1. Add 60.25 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave or overheat.
