Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
16.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Cream to yellow
Cultural Response
Inoculate and incubate at 35-37°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Indole production : (+)
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Indole production :(-)
Key
(+) : Positive reaction, formation of pink/red ring
(-) : Negative reaction, no formation of pink/red ring
Applications
Used for the differentiation of bacteria on the basis of indole production.
Composition
Ingredients
gm/lt.
Veg. Hydrolysate
10.00
Sodium chloride
5.00
DL- Tryptophan
1.00
Directions
1. Add 16.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave. at 121°C at 15 psi pressure for 15 minutes.
