Appearance (Colour)
White
Appearance (Form)
Crystalline powder
Solubility (Colour) 10% aq. solution
Clear, colourless solution
Assay
98.5%
Melting Point
167 - 170%
Chloride (CI)
Max 0.001%
Sulphate (SO4)
Max 0.01%
Iron (Fe)
Max 0.001%
Heavy Metals (Pb)
Max 0.001%
Acidity (CH3COOH)
Max 0.005%
Moisture
Max 0.5%
Reducing sugars
Max 0.1%
Sterility (Bacterial count)
No growth is observed at 35-37°C for bacteria and at 25°C for fungi for 14 days
Cultural Response
Inoculate and Incubate at 37°C for 18-24 hours in Phenol Red Broth Base using D-Mannitol Sterile (Gamma irradiated)
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Mannitol reaction : + (yellow colour), Gas : +
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Mannitol reaction : + (yellow colour)
Gas : +
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good, Mannitol reaction : - (no yellow colour), Gas : ---
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good, Mannitol reaction : + (yellow colour), Gas : --
Applications
For use in microbiological applications using D-Mannitol (gamma irradiated) and in Media Fill Runs in dry injectables.
