Appearance (Form)
Free flowing, homogeneous powder
Appearance (Colour)
Cream
Solubility
27.80 gm/lit
Solubility before autoclaving (Clarity)
Whitish buff, opalescent
Gel strength
Firm, comparable with 1.5% agar
pH
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Colour)
Whitish buff, opalescent
Cultural Response
Inoculate and incubate at 30°C for upto 5 days with the following organisms
Organism
Rhizobium leguminosarum ATCC1004
Inoculum (cfu) 10-100
Growth - Good
Organism
Rhizobium meliloti ATCC 9930
Inoculum (cfu) 10-100
Growth - Good
Applications
For the isolation and enumeration of soil microorganisms like Rhizobium species.
Composition
Ingredients
gms/lt.
Ingredients gm/lt.
Yeast extract
1.00
Mannitol
10.00
Dipotassium phosphate
0.50
Sodium chloride
0.10
Magnesium sulphate
0.20
Calcium carbonate
1.00
Agar
15.00
Directions
1. Add 27.80 gm powder to 1.0 litre of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
