Appearance (Colour)
Cream to yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
74.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent.
After addition of Egg Yolk Emulsion and CBI Supplement: Light yellow, very slightly opalescent.
Prepared Medium Appearance after autoclaving (Colour)
Basal Medium : Light yellow
Cultural Response
Inoculate and incubate at 35-37°C for upto 40-48 hours.
Organism
Clostridium botulinum ATCC 25763
Inoculum (cfu) 10-100
Growth : Good
Lecithinase : +ve
Applications
For the isolation, cultivation and differentiation of Clostridium botulinum from food samples.
Composition
Ingredients
gm/lt.
Pancreatic digest of casein
40.00
Yeast extract
5.00
Sodium chloride
2.00
Dextrose
2.00
Disodium phosphate
5.00
Magnesium sulphate
0.01
Agar
20.00
Directions
1. Add 74.00 gm powder to 900 ml of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50 °C.
5. Aseptically add 100 ml of egg yolk emulsion and 4 ml of CBI Supplement. (18871).
