Appearance (Form)
Free, flowing homogeneous powder
Solubility (Colour) 2% aq. solution
Light to medium amber, clear
pH (25°C)
7.0 ± 0.5 (2.0%)
Stability (After autoclaving)
Light to medium amber, clear
Loss on drying
<= 5.0%
Nitrogen content (N)
12.0 - 14.0%
NaCl
< 5.0%
Ash
< 11- 20%
Total Aerobic microbial count
(By Plate Count Method) : < 10,000 cfu/gm
Total Yeast and Mould count
(By Plate Count Method) : <= 100 cfu/gm
Proteose test
Not more than a slight, flocculent precipitate is formed. (Passes Test)
Cultural Response
Inoculate and incubate at 37°C for 18- 24 hours with the following organisms
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Escherichia coli ATCC 25922
Growth : Good, Result : Positive
Hydrogen Sulphide Production (1.0%)
Salmonella typhimurium ATCC 14028
Growth : Good, Result : Positive
Fermentable carbohydrates (2%)
Escherichia coli ATCC 25922
Growth : Good, Result : Negative
Acetylmethylcarbinol Production
Enterobacter aerogenes ATCC 13048
Growth : Good, Result : Positive
Applications
Enzymatic digest of protein, high in proteoses. Used in microbiological culture media and in bacterial toxin production and also in microbiological media for bacteria requiring varying nutritional properties.
