Appearance (Colour)
Light orange
Appearance (Form)
Free flowing, homogeneous powder
Solubility
24.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm comparable with 1.5% agar gel
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Yellowish orange
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good, Urease Production : +
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : Good, Urease Production : +
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good, Urease Production : -
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good, Urease Production : -
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Urease Production : -
Applications
Used for the detection of urease production by Proteus species and for identification of other members of Enterobacteriaceae.
Composition
Ingredients
gm/lt.
Veg. Peptone
1.00
Dextrose
1.00
Sodium chloride
5.00
Monopotassium phosphate
0.80
Disodium phosphate
1.20
Phenol Red
0.012
Agar
15.00
Directions
1. Add 24.00 gm powder to 950ml of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 50°C.
5. Under aseptic conditions, add 50ml of sterile 40% Urea solution (80192) and mix well.
6. Dispense into sterile test tubes.Allow tubes to solidify in a slanted position.
