Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
32.45 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Straw coloured opalesacent gel
Cultural Response
Inoculate and incubate at 35-37°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0 %
Colony colour : Purple to pink -magenta
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0 %
Colony colour : Colourless
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0 %
Colony colour : blue -green
Organism
Proteus vulgaris ATCC 12453
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0 %
Colony colour : light brown
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0 %
Colony colour : golden yellow
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Recovery : >=70.0 %
Colony colour : blue to purple,mucoid
Applications
A chromogenic medium used for the presumptive identification and differentiation of microorganisms causing urinary tract infections.
Composition
Ingredients
gm/lt.
Peptone
15.00
Chromogenic mixture
2.45
Agar
15.00
Directions
1. Add 32.45 gms powder in 1.0 liter distilled/purified water.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
